Download Membrane Glycoproteins. A Review of Structure and Function by R. Colin Hughes PDF

By R. Colin Hughes

Membrane Glycoproteins: A evaluate of constitution and serve as bargains with membrane glycoproteins present in organic structures. The e-book describes the constitution and biosynthesis of the glycoproteins with regards to identified or postulated capabilities in membranes. The textual content opens with an creation and a subject on detection and distribution of membrane glycoproteins. The publication then notes that the isolation of membrane glycoproteins introduced via the growth in study and know-how of membrane solubilization and purification of the soluble parts is now attainable. dialogue can be directed to glycoproteins as being vital parts of intracellular membranes, and never simply situated on cellphone surfaces. in the course of the structural research of glycoproteins produced by means of the secretory glands, research of human blood staff antigens is out there. Likewise, discoveries are made, explaining that lectins are important reagents in detecting the sort and numbers of glycoproteins stumbled on on mobile participants. Lectins are likewise being customary in exams for carbohydrate-containing components in membrane-mediated approaches. The metabolism, development keep an eye on, and phone floor reactions of membrane glycoproteins also are defined. The ebook can function a consultant for biologists, chemists, biochemists, and academicians attracted to the research of membranes or glycoproteins.

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Example text

Klenk and Uhlenbruck (1958) and Morawiecki (1964), for example, have performed the phenol extraction at 4 ° C or at room temperature rather than at 65—70 °C. T h e properties of the glycoprotein prepared in this way, in particular the molecular weight of the subunit, do not appear to be very different from the original preparation of Kathan et al. (1961). , 1966; Kathan and Adamany, 1967). T h e most highly purified preparations of the major erythrocyte glycoprotein derived by the phenol extraction method still retain fatty acids, presumably in the form of tightly bound glycolipids (Lisowska, 1968).

Similarly, Marchesi and Andrews (1971) were unable to remove glycolipids with A or B blood group activities from preparations of human erythrocyte glycoproteins by partition with cold phenol. , 1972) in their study of the major glycoprotein of human erythrocyte membranes. 1. , 1974). , 1972). T o my knowledge, no enzymically active membrane component has yet been solubilised with this reagent. The original description of the isolation of the major human erythrocyte glycoprotein is as follows. 05 M 25 mg m l of membrane protein.

High concentrations of these chaotropic anions disorder water, disrupt hydrophobic bonds near the surface of membrane structures and promote exposure of apolar groups. Several workers have used the method in order to solubilise membrane components. 1 M sucrose + 50 m M Tris—hydrochloric Isolation of Membrane Glycoproteins 41 acid, p H 8 , that was 2 M in sodium thiocyanate or perchlorate at 2 ° C for 5—10 min. Solubilised proteins were present in the supernatant after centrifugation at 105 000 g for 2 h.

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